CYTOOchips™ Starter's FN x18

The CYTOOchip is a 19.5x19.5 mm coverslip with micropatterns transferred by photolithography on a high quality and low fluorescence borosilicate glass. The CYTOOchips are compatible with all inverted microscopes. Grid coordinates are printed on the underside of the chip and enable the user to return to the exact same cell/micropattern over time. These chips represent an ideal choice for high-resolution live cell imaging when combined with CYTOOchambers.

Product specification

  • Reference:
    10-900-10-18
    Pattern:
    Multiple
    Size:
    Multiple
    Coating:
    Fibronectin
    Quantity
    Set of 18

Publications

Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis
Hurtado L / Caballero C / Gavilan MP / Cardenas J / Bornens M / Rios RM
26 May 2014
Keywords:
pericentrosomal Golgi ribbon organization, AKAP450 N-terminal fragments, microtubule nucleation, centrosomal activity, Golgi apparatus positioning, directional cell migration, ciliogenesis, Y-shaped micropatterned coverslips, cell movement

J. Cell Biol. 2011;193(5):917–933.

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Spatial organization of the extracellular matrix regulates cell-cell junction positioning
Tseng Q / Duchemin-Pelletier E / Deshiere A
24 May 2014
Keywords:
intercellular junctions, cell line, extracellular matrix

Proc. Natl. Acad. Sci. U.S.A. 2012;109(5):1506–1511.

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A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells
Burnette DT / Shao L / Ott C / Pasapera AM / Fischer RS / Baird MA / Der Loughian C / Delanoe-Ayari H / Paszek MJ / Davidson MW / Betzig E / Lippincott-Schwartz J
26 May 2014
Keywords:
Live-cell microscopy, Contractility, 3D structures

J Cell Biol. 2014 Apr 14;205(1):83-96. doi: 10.1083/jcb.201311104. Epub 2014 Apr 7.

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A Mechanical Checkpoint Controls Multicellular Growth through YAP/TAZ Regulation by Actin-Processing Factors
Aragona M / Panciera T / Manfrin A
26 May 2014
Keywords:
YAP/TAZ, oncology, cell shape, cell proliferation

Cell. 2013; Aug 15 [epub ahead of print].

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A method to recapitulate early embryonic spatial patterning in human embryonic stem cells.
Warmflash A / Sorre B / Etoc F / Siggia ED / Brivanlou AH.
18 July 2014
Keywords:
hESC, Assay, stem cells, Developmental Biology, Maturation, Differentiation, Gastrulation, Spacial Patterning

Nat Methods. 2014 Jun 29. doi: 10.1038/nmeth.3016

 

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A Novel Organelle Map Framework for High-Content Cell Morphology Analysis in High Throughput
Schauer K / Grossier J-P / Duong T
26 May 2014
Keywords:
organelle map, high content screening, high throughput screening

J Biomol Screen. 2013; Aug 16 [epub ahead of print].

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A review of microfabrication and hydrogel engineering for micro-organs on chips
Verhulsel M / Vignes M / Descroix S / Malaquin L / Vignjevic DM / Viovy J-L
26 May 2014
Keywords:
tissue engineering, 3D cell culture

Biomaterials. 2013; Dec 04 [epub ahead of print].

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Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity
Théry M / Racine V / Piel M
26 May 2014
Keywords:
Cell polarity, extracellular matrix, morphogenesis, cell normalization, organelle positioning

Proc. Natl. Acad. Sci. U.S.A. 2006;103(52):19771–19776.

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Arl8 and SKIP act together to link lysosomes to kinesin-1
Rosa-Ferreira C / Munro S
26 May 2014
Keywords:
lysosomes, kinesin

Dev. Cell. 2011;21(6):1171–1178.

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Assessing Kinetics from Fixed Cells Reveals Activation of the Mitotic Entry Network at the S/G2 Transition
Karen Akopyan / Helena Silva Cascales / Elvira Hukasova / Adrian T. Saurin / Erik Müllers / Himjyot Jaiswal / Danielle A.A. Hollman / Geert J.P.L. Kops / René H. Medema / Arne Lindqvist
26 May 2014
Keywords:
cell cycle, cell division, mitosis

Molecular Cell - 27 February 2014

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Carminomycin I is an apoptosis inducer that targets the Golgi complex in clear cell renal carcinoma cells
Woldemichael GM / Turbyville TJ / Linehan WM / McMahon JB
26 May 2014
Keywords:
high-throughput screening assays, clear cell renal cell carcinoma (CCRCC), Von Hippel-Lindau (VHL) tumor suppressor gene, Golgi sequestration, P-gp-mediated localization of anticancer compounds, disk and L shaped fibronectin micropattern

 Cancer Res. 2011;71(1):134–142.

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CD81 regulates cell migration through its association with Rac GTPase
Tejera E / Rocha-Perugini V / López-Martín S
26 May 2014
Keywords:
cell migration, tetraspanin, Rho GTPases; focal adhesions

Mol. Biol. Cell. 2012; Dec 05 [epub ahead of print].

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Cell confinement controls centrosome positioning and lumen initiation during epithelial morphogenesis
Rodrigues-Fraticelli AE / Auzan M / Alonso MA / Bornens M / Martin-Belmonte F
26 May 2014
Keywords:
morphogenesis, cell normalization

J Cell Biol. 2012;198(6):1011-1023.

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Cell distribution of stress fibres in response to the geometry of the adhesive environment
Théry M / Pépin A / Dressaire E / Chen Y / Bornens M
26 May 2014
Keywords:
pattern geometry, apices of cell shape, stress fibres, focal adhesions, tractional forces, cell shape, cell surface, cell adhesion, cell line

Cell Motil. Cytoskeleton. 2006;63(6):341–355

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Cell shape and contractility regulate ciliogenesis in cell cycle-arrested cells
Pitaval A / Tseng Q / Bornens M / Théry M
26 May 2014
Keywords:
Primary cilium, cilia, cell shape, Cell polarity

J. Cell Biol. 2010;191(2):303–312.

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Cells in tight spaces: the role of cell shape in cell function
Shah JV
26 May 2014
Keywords:
cell shape, cell function, cell physiological phenomena, ciliogenesis

J. Cell Biol. 2010;191(2):233–236.

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Chapter 7 - Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3D Systems
Rodríguez-Fraticelli AE / Martin-Belmonte F
26 May 2014
Keywords:
3D cell culture, morphogenesis

Methods in Cell Biology.Vol Volume 118. Methods for Analysis of Golgi Complex Function. Academic Press; 2013:105–123.

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Chapter 7 - Studying Mitochondria and Microtubule Localization and Dynamics in Standardized Cell Shapes
Pelikan A / Sillibourne J / Miserey-Lenkei S / Carlier-Grynkorn F / Goud B / Tran PT
26 May 2014
Keywords:
mitochondria, microtubules, cell shape

Methods in Cell Biology. Vol 115. Academic Press; 2013:97–108.

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Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation
Kiyomitsu T / Cheeseman IM
26 May 2014
Keywords:
spindle orientation on L-patterned fibronectin-coated micropatterns, RNAi experiments on patterned coverslips, dynein-dynactin localization, Polo-like kinase 1, NuMA, LGN, RanGTP

Nat. Cell Biol. 2012;14(3):311–317.

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Cortical Dynein and Asymmetric Membrane Elongation Coordinately Position the Spindle in Anaphase
Kiyomitsu T / Cheeseman IM
26 May 2014
Keywords:
cell division, dynein, spindle orientation

Cell. 2013;154(2):391–402.

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Cortical dynein is critical for proper spindle positioning in human cells
Kotak S / Busso C / Gönczy P
26 May 2014
Keywords:
golgi, spindle positioning, dynein

J Cell Biol. 2012;199(1):97.

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Directional Persistence of Migrating Cells Requires Kif1C-Mediated Stabilization of Trailing Adhesions
Theisen U / Straube E / Straube A
26 May 2014
Keywords:
cell migration, Kif1C, transport kinesin, directional migration

Dev Cell. 2012;23(6):1153–1166.

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Dynamics of Cell Shape and Forces on Micropatterned Substrates Predicted by a Cellular Potts Model
Philipp J. Albert and Ulrich S. Schwarz
13 November 2014
Keywords:
Forces, dynamics, biophysics, modelling, cell behavior, spreading, prediction

Biophys J. 2014 Jun 3;106(11):2340-52. doi: 10.1016/j.bpj.2014.04.036.

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Experimental and theoretical study of mitotic spindle orientation
Théry M / Jiménez-Dalmaroni A / Racine V / Bornens M / Jülicher F
26 May 2014
Keywords:
mitotic spindle orientation, cell microenvironment geometry, cortical cues, spindle pole, symmetric/ asymmetric orientation

Nature. 2007;447(7143):493–496.

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FAK transduces extracellular forces that orient the mitotic spindle and control tissue morphogenesis
Nicoletta I. Petridou & Paris A. Skourides
13 November 2014
Keywords:
mitotic spindle, morphogenesis, FAK, development, embryo, mitosis

Nature Communications 5, Article number: 5240 doi:10.1038/ncomms6240

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Filamin A-Hinge region 1-EGFP : a novel tool for tracking the cellular function of filaminA in real-time
Planagumà J / Minsaas L / Pons M / Myhren L / Garrido G / Aragay AM
26 May 2014
Keywords:
cellular function, Filamin A, cell normalization

Plos One. 2012;7(8):e40864.

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Filamin A-hinge region 1-EGFP: a novel tool for tracking the cellular functions of Filamin A in real-time
Planagumà J / Minsaas L / Pons M / Myhren L / Garrido G / Aragay AM
26 May 2014
Keywords:
Filamin A, cell surface, cell physiological phenomena

PLoS ONE. 2012;7(8):e40864.

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Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure
Diogo Mosqueira / Stefania Pagliari / Koichiro Uto / Mitsuhiro Ebara / Sara Romanazzo / Carmen Escobedo-Lucea / Jun Nakanishi / Akiyoshi Taniguchi / Ornella Franzese / Paolo Di Nardo / Marie José Goumans / Enrico Traversa / Perpetua Pinto-do-Ó / Takao Aoyagi / and Giancarlo Forte
27 May 2014
Keywords:
adult cardiac progenitor cell, substrate nanotopography, mechano-transduction; cardiac differentiation; YAP/TAZ

ACS Nano, 2014, 8 (3), pp 2033–2047 Publication Date (Web): February 2, 2014

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Human Bone Marrow Mesenchymal Stem Cells Regulate Biased DNA Segregation in Response to Cell Adhesion Asymmetry
Freida D / Lecourt S / Cras A
26 May 2014
Keywords:
stems cells, cell division, cell adhesion

Cell Rep. 2013;Oct 17 [epub ahead of print].

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Improved visualization and quantitative analysis of drug effects using micropatterned cells
Degot S / Auzan M / Chapuis V
26 May 2014
Keywords:
Reference Cell, drug profiling, quantitative cell analysis, cell adhesion, cytoskeleton, drug evaluation

J Vis Exp. 2010;(46):pii: 2514.

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Intercellular and extracellular mechanotransduction in cardiac myocytes
Kresh JY / Chopra A
26 May 2014
Keywords:
adult cardiomyocytes, extracellular matrix, mechanical determinants, cytoskeletal organization, shape remodelling, sarcomere/myofibrillar architectural topography, cell shape-dependent functions, myopathies, myocardial infarctus, heart failure, reverse remodeling, disk-, H-, Y-, crossbow-shaped micropatterns, sarcomeric alpha-actinin.

Pflugers Arch. 2011;462(1):75–87.

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Intracellular Electric Field and pH Optimize Protein Localization and Movement
Cunningham J / Estrella V / Lloyd M / Gillies R / Frieden BR / Gatenby R
26 May 2014
Keywords:
EGFR/ MAPK pathway, intracellular electric field

 PLoS ONE. 2012;7(5):e36894.

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Lack of a Functional VHL Gene Product Sensitizes Renal Cell Carcinoma Cells to the Apoptotic Effects of the Protein Synthesis Inhibitor Verrucarin A
Woldemichael GM / Turbyville TJ / Vasselli JR / Linehan WM / McMahon JB
26 May 2014
Keywords:
cell proliferation, apoptosis, cytotoxic stress

Neoplasia. 2012;14(8):771–777.

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LNK (SH2B3) is a key regulator of integrin signaling in endothelial cells and targets α-parvin to control cell adhesion and migration
Devallière J / Chatelais M / Fitau J
26 May 2014
Keywords:
cell migration, cell adhesion, focal adhesions

FASEB J. 2012;26(6):2592–2606.

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Long-Lasting Effect of Perinatal Exposure to L-tryptophan on Circadian Clock of Primary Cell Lines Established from Male Offspring Born from Mothers Fed on Dietary Protein Restriction
Nascimento E / Guzman-Quevedo O / Delacourt N
26 May 2014
Keywords:
circadian clock, undernutrition, primary cell line, L-tryptophan

PLoS ONE. 2013;8(2):e56231.

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MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis
Samora CP / Mogessie B / Conway L / Ross JL / Straube A / McAinsh AD
26 May 2014
Keywords:
mitotic spindle orientation, H pattern, cell division, cell line, mitosis, mitotic spindle apparatus, microtubules

Nat. Cell Biol. 2011;13(9):1040–1050.

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Mechanical control of epithelial lumen formation
Rodríguez-Fraticelli AE / Martin-Belmonte F
26 May 2014
Keywords:
epithelial cells, lumen, ciliogenesis, centrosome, morphogenesis

Small GTPases. 2013;4(2).

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Mechanism of the Ca2+-Dependent Interaction between S100A4 and Tail Fragments of Nonmuscle Myosin Heavy Chain IIA.
Badyal SK / Basran J / Bhanji N
26 May 2014
Keywords:
epidermoid carcinoma A431/SIP1 cells constitutively expressing the GFP-tagged heavy chain of non-muscle myosin IIA, medium Y-shaped fibronectin micropattern

 J Mol Biol. 2011;405(4-3):1004–1026.

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Mechanisms of spindle positioning: cortical force generators in the limelight
Kotak S / Gönczy P
26 May 2014
Keywords:
cell division, spindle, microtubules

Curr Opin Cell Biol. 2013;Aug 16 [epub ahead of print].

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Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes
Kwon M / Godinho SA / Chandhok NS
24 May 2014
Keywords:
tumor cells, Micropatterns, H shape, Y shape, cell division, neoplasms, cell adhesion, cell line, cell shape, Cell polarity

 Genes Dev. 2008;22(16):2189–2203.

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Microfluidic cell trap array for controlled positioning of single cells on adhesive micropatterns
Lin L / Chu Y-S / Thiery JP / Lim CT / Rodriguez I
26 May 2014
Keywords:
cell shape, Cell polarity, cell function, cell migration, single cells

Lab Chip. 2013;13(4):714–721.

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Micropatterned substrates coated with neuronal adhesion molecules for high-content study of synapse formation
Czöndör K / Garcia M / Argento A
26 May 2014
Keywords:
neuroscience, neuron cell, cell adhesion, morphogenesis

Nat Commun. 2013;4(2252).

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Micropatterning as a tool to decipher cell morphogenesis and functions
Théry M.
24 May 2014
Keywords:
cell microenvironment, cell architecture, engineered micropatterns, cell morphogenetic processes, micropattern shape, actin, microtubule, cell differentiation, Cell polarity, cell shape, cell proliferation

J. Cell. Sci. 2010;123(24):4201–4213.

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Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns
Stiles JM / Pham R / Rowntree RK
26 May 2014
Keywords:
endothelial cells, cell morphology, cytoskeleton, genomics

FEBS J. 2013; June 27 [epub ahead of print].

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Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation
Guenin-Macé L / Veyron-Churlet R / Thoulouze M-I
26 May 2014
Keywords:
immunology, Buruli, mycolactone

J Clin Invest. 2013; Mar 15 [epub ahead of print].

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Myosin-Va and Dynamic Actin Oppose Microtubules to Drive Long-Range Organelle Transport
Richard D. Evans / Christopher Robinson / Deborah A. Briggs / David J. Tooth / Jose S. Ramalho / Marta Cantero / Lluis Montoliu / Shyamal Patel / Elena V. Sviderskaya / and Alistair N. Hume
13 November 2014
Keywords:
microtubule, actin, Myosin, Melanosomes, Melanocytes, Reference Cell, Organelles, Trafficking

Curr Biol. 2014 Aug 4;24(15):1743-50. doi: 10.1016/j.cub.2014.06.019. Epub 2014 Jul 24.

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Patterning Pluripotent Stem Cells at a Single Cell Level
Pryzhkova MV / Harris GM / Ma S / Jabbarzadeh E
26 May 2014
Keywords:
stem cells, vitronectin, extracellular matrix

J Biomater Tissue Eng. 2013;3(4):461–471

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Phosphatidylinositol 5-phosphate regulates invasion through binding and activation of Tiam1
Julien Viaud / Frederic Lagarrigue / Damien Ramel / Sophie Allart / Gaetan Chicanne / Laurie Ceccato / Delphine Courilleau / Jean-Marie Xuereb / Olivier Pertz / Bernard Payrastre & Frederique Gaits-Iacovoni
13 November 2014
Keywords:
Phosphoinositides, oncology, RPE-1, MEF, invasion, bacteriology, lymphoma, FRET, endosome

Nat Commun. 2014 Jun 6;5:4080. doi: 10.1038/ncomms5080.

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Practical course on “Imaging infection: from single molecules to animals.”
Guenot M / Racz P
26 May 2014
Keywords:
Fluorescence microscopy, bioluminescent imaging, High Throughput microscopy, super-resolution microscopy : TIRF, PALM/STORM, in vivo imaging, PETSCAN, CT-SCAN, FRET, live cell imaging, image analysis, photobleaching, phototoxicity

Microbes Infect. 2012; Nov 02 [epub ahead of print].

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Probabilistic density maps to study global endomembrane organization
Schauer K / Duong T / Bleakley K / Bardin S / Bornens M / Goud B.
24 May 2014
Keywords:
endomembranes, small G proteins, cell line, intracellular membranes

Nat. Methods. 2010;7(7):560–566.

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Rab GTPase Mediated Procollagen Trafficking in Ascorbic Acid Stimulated Osteoblasts
Nabavi N / Pustylnik S / Harrison RE
26 May 2014
Keywords:
osteoblasts, protein trafficking

PLoS ONE. 2012;7(9):e46265.

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Reconstituting actomyosin-dependent mechanosensitive protein complexes in vitro
Ciobanasu C / Faivre B / Le Clainche C
08 January 2015
Keywords:
mechanosensitivity, ABP, actomyosin, cytoskeleton, vinculin, talin, TIRF

Nat Protoc. 2015 Jan;10(1):75-89. doi: 10.1038/nprot.2014.200. Epub 2014 Dec 11.

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Reconstitution of in vivo macrophage-tumor cell pairing and streaming motility on one-dimensional micro-patterned substrates
Sharma V / Beaty B / Patsialou A
26 May 2014
Keywords:
cell migration, oncology

IntraVital. 2012;1(1):77-85.

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Reprogramming cell shape with laser nano-patterning.
Vignaud T / Galland R / Tseng Q / Blanchoin L / Colombelli J / Théry M.
24 May 2014
Keywords:
cell shape, actin network reprogramming, dynamic cellular responses, laser nano-patterning, Cell polarity

J. Cell. Sci. 2012;125(9):2134–2140.

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Role of YAP/TAZ in mechanotransduction
Dupont S / Morsut L / Aragona M
24 May 2014
Keywords:
mechanotransduction, mesenchymal stem cells, cell differentiation, cell line, cell shape, cytoskeleton

Nature. 2011;474(7350):179–183.

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Spatial control of membrane receptor function using ligand nanocalipers
Alan Shaw / Vanessa Lundin / Ekaterina Petrova / Ferenc Fördős / Erik Benson / Abdullah Al-Amin / Anna Herland / Andries Blokzijl3 / Björn Högberg & Ana I Teixeira
13 November 2014
Keywords:
Signaling, receptor, ligand, nanocaliper, breast cancer, membrane, ephrin-A5, invasion, presentation

Nat Methods. 2014 Aug;11(8):841-6. doi: 10.1038/nmeth.3025. Epub 2014 Jul 6.

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Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL
Kitagawa D / Kohlmaier G / Keller D
22 May 2014
Keywords:
microcephaly, mitotic spindle orientation

J. Cell. Sci. 2011;124(22):3884–3893.

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Standardized mitochondrial analysis gives new insights into mitochondrial dynamics and OPA1 function. Int
Chevrollier A / Cassereau J / Ferré M
22 May 2014
Keywords:
mitochondrial dynamics, mitochondrial disorder diagnostics, OPA1, neurodegenerative diseases, mitochondria

J. Biochem. Cell Biol. 2012;44(6):980–988.

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The Bin/Amphiphysin/Rvs (BAR) Domain Protein Endophilin B2 Interacts with Plectin and Controls Perinuclear Cytoskeletal Architecture
Vannier C / Pesty A / San-Roman MJ / Schmidt AA
26 May 2014
Keywords:
cytoskeleton, nucleus positioning

J Biol Chem. 2013;288(38):27619–27637.

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The extracellular matrix guides the orientation of the cell division axis.
Théry M / Racine V / Pépin A
22 May 2014
Keywords:
cell fate, cell division, cell shape, cell membrane, Cell polarity, actin dynamics, average distribution of actin-binding proteins

Nat. Cell Biol. 2005;7(10):947–953.

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The interaction between nesprins and sun proteins at the nuclear envelope is critical for force transmission between the nucleus and cytoskeleton.
Lombardi ML / Jaalouk DE / Shanahan CM / Burke B / Roux KJ / Lammerding J.
22 May 2014
Keywords:
cell line, cytoskeleton, mechanotransduction, nuclear envelope, nucleo-cytoskeletal coupling, force transmission, LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, intracellular forces, nuclear positioning, cell polarization, muscular dystrophies, cardiomyopathies, crossbow-shaped micropatterns

J. Biol. Chem. 2011;286(30):26743–26753.

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The role of RPGR in cilia formation and actin stability
Gakovic M / Shu X / Kasioulis I / Carpanini S / Moraga I / Wright AF
22 May 2014
Keywords:
cilia, ciliogenesis, retinal dystrophy, actins, cytoskeleton, cell cycle, cell division, cell line, cell movement, cell shape, epithelial cells, eye proteins, focal adhesions, organogenesis

Hum. Mol. Genet. 2011;20(24):4840–4850

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β1- and αv-class integrins cooperate to regulate myosin II during rigidity sensing of fibronectin-based microenvironments
Schiller HB / Hermann M-R / Polleux J
26 May 2014
Keywords:
integrins, cell adhesion, cell contractility, cell motility, traction force

Nat Cell Biol. 2013; May 26 [epub ahead of print] .

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Shipment and information

In order to guarantee a constant quality as well as a long shelf life for our products, CYTOOchips are packaged by 6 in a blister pack sealed in an aluminum bag under inert gas.

The average turnaround time for production is 4-5 weeks.

The CYTOOchips should be stored at 4°C and used before the expiry date indicated on the packaging (6 months from the production date).

Stability is guaranteed for at least 3 months from the date of shipment when stored at 4°C in its original packaging.