Human bile canaliculi derived from HepG2-C3A cell line

By combining the more liver-like cell line HepG2-C3A with specific micropatterns, we were able to improve the maturity level of a standard cell line by creating small multi-cellular structures each containing bile canaliculi with a higher level of CYP3A4 activity. Isoniazid hepatotoxicity, linked to its reactive metabolite, was detected using this model.


Increased fusion and area of bile canaliculi

Increased CYP3A4 metabolic activity


Phospholipidosis (LipidTOX™ Invitrogen)


More sensitive than HepG2 and PHH, parallel screening of cytotoxicity

Our hepatocyte cellular model can be in-licensed should you wish to further develop novel HCA compatible read-outs and assays. CYTOO also offers custom cellular model and cell-based assay development through research contracts.

For more information on our cellular models and Cell-Based Assays, please contact us. For business options and terms, click here.