3 Bethesda MetroCenter
Bethesda, MD 20814
Phone: (001) 301 525 5262
By combining the more liver-like cell line HepG2-C3A with specific micropatterns, we were able to improve the maturity level of a standard cell line by creating small multi-cellular structures each containing bile canaliculi with a higher level of CYP3A4 activity. Isoniazid hepatotoxicity, linked to its reactive metabolite, was detected using this model.
Increased fusion and area of bile canaliculi
Increased CYP3A4 metabolic activity
Phospholipidosis (LipidTOX™ Invitrogen)
More sensitive than HepG2 and PHH, parallel screening of cytotoxicity
Our hepatocyte cellular model can be in-licensed should you wish to further develop novel HCA compatible read-outs and assays. CYTOO also offers custom cellular model and cell-based assay development through research contracts.