By combining the more liver-like cell line HepG2-C3A with specific micropatterns, we were able to improve the maturity level of a standard cell line by creating small multi-cellular structures each containing bile canaliculi with a higher level of CYP3A4 activity. Isoniazid hepatotoxicity, linked to its reactive metabolite, was detected using this model.

Features

Increased fusion and area of bile canaliculi

Increased CYP3A4 metabolic activity

Applications

Phospholipidosis (LipidTOX™ Invitrogen)

Advantages

More sensitive than HepG2 and PHH, parallel screening of cytotoxicity

Our hepatocyte cellular model can be in-licensed should you wish to further develop novel HCA compatible read-outs and assays. CYTOO also offers custom cellular model and cell-based assay development through research contracts.

For more information on our cellular models and Cell-Based Assays, please contact us. For business options and terms, click here.